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( a ) Comparative representation of TrkAIII and fs-TrkA exon structures. ( b ) TrkAIII qRT-PCR Assay 1 and 2 strategies, illustrating TrkAIII specificity provided by the TaqMan probe and by the antisense primer (R), respectively.

Journal: Cancers

Article Title: A TaqMan-Based qRT-PCR Assay for Accurate Evaluation of the Oncogenic TrkAIII Splice Variant in Tumor cDNAs

doi: 10.3390/cancers17030471

Figure Lengend Snippet: ( a ) Comparative representation of TrkAIII and fs-TrkA exon structures. ( b ) TrkAIII qRT-PCR Assay 1 and 2 strategies, illustrating TrkAIII specificity provided by the TaqMan probe and by the antisense primer (R), respectively.

Article Snippet: Assay primers and probes were selected from NTRK1/TrkA (NM_001012331.1) and TrkAIII (GenBank: OP866787.1) sequences using Custom TaqMan ® Assay Design Tool software ( https://www.thermofisher.com/order/custom-genomic-products/tools/genotyping/ , accessed on 7 March 2023) and were synthesized by Thermo Fisher Scientific (Waltham, MA, USA).

Techniques: Quantitative RT-PCR